Duties: The Research Specialist will manage research projects and perform a broad range of experiments related with evaluating cellular responses generated by glucocorticoids and cAMP-elevating agents, encompassing cell and molecular biology, tissue culture and animal work techniques. Primary responsibilities will include culturing different lung cell types (both primary cells and cell lines), performing human and murine Precision Cut Lung Slice (PCLS) experiments, animal work (colony maintenance & genotyping, necroscopy, and drug administration [gavage, iv., ip., sc., it. or in.]), performing immunological assays (immunofluorescent staining, ELISA, western blots, immunoprecipitations, and flow cytometry/FACS), and molecular biology techniques (PCR & real time-PCR techniques, DNA and mRNA sample preparation, plasmid DNA preparation, DNA subcloning, siRNA/shRNA transfections, Adenovirus/Lentivirus production, and in situ hybridizations). Additional responsibilities will include maintaining equipment; keeping accurate records and notes; ordering lab supplies; working knowledge of MS Office to keep inventories of experimental reagents and equipment; data analysis and general lab duties. The Research Specialist will act in a lead capacity and will orient/train others in the lab.
Qualifications: A Bachelor's Degree in one of the following fields: biology, cell biology, biochemistry, molecular biology, or other related fields and 3 years to 5 years of laboratory experience or equivalent combination of education and experience is required. Expertise in performing basic scientific assays including: RNA/DNA extraction, and protein preparation, PCR & real time-PCR, western blot analysis, ELISA, flow cytometry/FACS are essential. Familiarity with basic concepts of cell, cell-cell signaling, and molecular biology are required. A strong background in cell and molecular biology, tissue culture, and experience in handling, breeding, and/or surgery with mice or rats is a prerequisite for this position. Additional training will be provided as needed. Strong verbal and written communication skills, ability to work independently and as part of a team are required. Candidates with miRNA experience and/or pulmonary research experience are preferred. Position is contingent upon funding.
Reference Number: 40-20117
Salary Grade: 026
Employment Type: Exempt
Org: DM-Pulmonary Airways Biology Initiative
Special Requirements: Background Check Required
Job Family: I-Technical/Professional Research